Kelly T J, Holt P R, Wu A L
Am J Clin Nutr. 1980 May;33(5):1033-40. doi: 10.1093/ajcn/33.5.1033.
The effect of 2 to 3 weeks of 73% sucrose feeding and intestinal sucrose infusion upon intestinal lipoprotein formation was studied in mesenteric lymph cannulated female rats. Lymph lipoprotein fractions were analyzed for lipid and apoprotein content and were compared to chow-fed control rats. The sucrose regimen increased mesenteric lymph triglyceride output by 54%, the increase being confined to the very low-density lipoprotein fraction. Sucrose infusion in chow-fed control rats did not increase lymph triglyceride transport when compared to infusion of a glucose polymer. Unesterified cholesterol output in whole lymph was stimulated by sucrose by 76%. Both the d greater than 1.006 and d less than 1.006 g/ml fractions of lymph were found to have an increase in unesterified cholesterol output. Since no difference in the chemical composition of very low-density lipoproteins in the two groups of animals was detected, sucrose appeared to increase the number of very low-density lipoprotein particles secreted by the intestine. Sucrose did not alter the relative proportions of apo A1, apo A4, apo ARP, and total C peptides present in very low-density lipoproteins. However, differences in the proportion of apo C subunits after sucrose were detected by isoelectric focusing with a pronounced increase in the apo CIII0 peak. The present studies demonstrate that the intestine participates in changes in lipoprotein formation and metabolism that accompany experimental sucrose induced hyperlipidemia.
在肠系膜淋巴管插管的雌性大鼠中，研究了2至3周73%蔗糖喂养和肠道蔗糖输注对肠道脂蛋白形成的影响。分析了淋巴脂蛋白组分的脂质和载脂蛋白含量，并与喂食普通饲料的对照大鼠进行比较。蔗糖喂养方案使肠系膜淋巴甘油三酯输出量增加了54%，这种增加仅限于极低密度脂蛋白组分。与输注葡萄糖聚合物相比，在喂食普通饲料的对照大鼠中输注蔗糖并没有增加淋巴甘油三酯的转运。蔗糖使全淋巴中未酯化胆固醇的输出量增加了76%。发现淋巴中密度大于1.006和密度小于1.006 g/ml的组分的未酯化胆固醇输出量均增加。由于在两组动物中未检测到极低密度脂蛋白的化学组成有差异，蔗糖似乎增加了肠道分泌的极低密度脂蛋白颗粒的数量。蔗糖没有改变极低密度脂蛋白中载脂蛋白A1、载脂蛋白A4、载脂蛋白ARP和总C肽的相对比例。然而，通过等电聚焦检测到蔗糖处理后载脂蛋白C亚基比例存在差异，载脂蛋白CIII0峰明显增加。目前的研究表明，肠道参与了伴随实验性蔗糖诱导的高脂血症的脂蛋白形成和代谢变化。
