Bird M M
Anat Embryol (Berl). 1980;159(1):115-23. doi: 10.1007/BF00299260.
Embryonic chick corpus striatum neurons were dissociated and maintained on liver feeder layers in culture. Although some large dark-cored vesicles were present in many nerve processes and presynaptic boutons they were substantially less numerous than chick spinal cord neurons grown under identical conditions. Paraformaldehyde-induced fluorescence, although observed in a few culture batches in aggregates of corpus striatum neurons, was otherwise absent and no decisive evidence was obtained to suggest that fluorescent corpus striatum neurons were commonly developed on liver feeder layers in culture. Microtubules filled most cell bodies and nerve processes, and extended well into synaptic boutons often approaching the active zones. They were much more abundant in cultures of corpus striatum than in comparable spinal cord preparations and formed the principal organelle of many nerve fibres. These differences between chick spinal cord and corpus striatum neurons are both interesting and difficult to interpret. It is possible that fewer appropriate cholinergic neurons are available for transformation into adrenergic neurons within the corpus striatum, and that excessive numbers of dark-cored vesicles indicate only a greatly increased rate of acetylcholine production and storage.
将鸡胚纹状体神经元解离，并培养于肝脏饲养层上。尽管在许多神经突起和突触前终扣中存在一些大的深色核心囊泡，但与在相同条件下培养的鸡脊髓神经元相比，其数量要少得多。虽然在一些培养批次的纹状体神经元聚集体中观察到了多聚甲醛诱导的荧光，但在其他情况下则未观察到，并且没有获得决定性证据表明荧光纹状体神经元在培养的肝脏饲养层上普遍发育。微管充满了大多数细胞体和神经突起，并延伸到突触终扣中，常常靠近活性区。它们在纹状体培养物中比在可比的脊髓制剂中丰富得多，并构成了许多神经纤维的主要细胞器。鸡脊髓和纹状体神经元之间的这些差异既有趣又难以解释。有可能在纹状体内可转化为肾上腺素能神经元的合适胆碱能神经元较少，并且大量的深色核心囊泡仅表明乙酰胆碱产生和储存的速率大大增加。
