非神经元细胞对解离的交感神经元培养物中儿茶酚胺和乙酰胆碱合成及积累的影响。
The influence of non-neuronal cells on catecholamine and acetylcholine synthesis and accumulation in cultures of dissociated sympathetic neurons.
作者信息
Patterson P H, Chun L L
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3607-10. doi: 10.1073/pnas.71.9.3607.
Abstract
The effects of several non-neuronal cell types on neurotransmitter synthesis in cultures of dissociated sympathetic neurons from the new-born rat were studied. Acetylcholine synthesis from radioactive choline was increased 100- to 1000-fold in the presence of non-neuronal cells from sympathetic ganglia. This increase was roughly dependent on the number of ganglionic non-neuronal cells present. The effect did not appear to be due to an increased plating efficiency of neurons, since the non-neuronal cells were capable of increasing acetylcholine synthesis after only 48-hr contact with neurons that had been previously grown without non-neuronal cells for 2 weeks. C6 rat glioma cells were also able to stimulate acetylcholine synthesis, but 3T3 mouse fibroblast cells had little or no effect. None of the non-neuronal cell types synthesized detectable acetylcholine in the absence of the neurons. The ganglionic non-neuronal cells had no significant effect on catecholamine synthesis (which occurs in the absence of non-neuronal cells).
摘要
研究了几种非神经元细胞类型对新生大鼠离体交感神经元培养物中神经递质合成的影响。在存在来自交感神经节的非神经元细胞的情况下，由放射性胆碱合成的乙酰胆碱增加了100至1000倍。这种增加大致取决于存在的神经节非神经元细胞的数量。这种作用似乎不是由于神经元接种效率的提高，因为非神经元细胞在仅与先前在无非神经元细胞的情况下生长了2周的神经元接触48小时后，就能增加乙酰胆碱的合成。C6大鼠胶质瘤细胞也能够刺激乙酰胆碱的合成，但3T3小鼠成纤维细胞几乎没有影响。在没有神经元的情况下，没有一种非神经元细胞类型能合成可检测到的乙酰胆碱。神经节非神经元细胞对儿茶酚胺合成（在没有非神经元细胞的情况下发生）没有显著影响。
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本文引用的文献
1
Essential role of the nerve growth factor in the survival and maintenance of dissociated sensory and sympathetic embryonic nerve cells in vitro.神经生长因子在体外对解离的胚胎感觉和交感神经细胞存活及维持方面的重要作用。
Dev Biol. 1963 Mar;6:653-9. doi: 10.1016/0012-1606(63)90149-0.
2
Dissociation, fractionation and culture of chick embryo sympathetic ganglionic cells.鸡胚交感神经节细胞的解离、分级分离和培养
J Neurocytol. 1972 Oct;1(3):211-21. doi: 10.1007/BF01099934.
3
Surface movements during the growth of single explanted neurons.单个外植神经元生长过程中的表面运动。
Proc Natl Acad Sci U S A. 1970 Apr;65(4):905-10. doi: 10.1073/pnas.65.4.905.
4
Isolated dorsal root ganglion neurons in culture: cytological maturation and extension of electrically active processes.培养的孤立背根神经节神经元：细胞学成熟与电活动过程的延伸
J Neurobiol. 1972;3(2):111-51. doi: 10.1002/neu.480030203.
5
Radiochemical micro assays for the determination of choline acetyltransferase and acetylcholinesterase activities.用于测定胆碱乙酰转移酶和乙酰胆碱酯酶活性的放射化学微量分析。
Biochem J. 1969 Nov;115(3):465-72. doi: 10.1042/bj1150465.
6
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7
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8
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