抗坏血酸对大鼠平滑肌细胞胶原蛋白和弹性蛋白的性质及生成的影响。
The effect of ascorbic acid on the nature and production of collagen and elastin by rat smooth-muscle cells.
作者信息
de Clerck Y A, Jones P A
出版信息
Biochem J. 1980 Jan 15;186(1):217-25. doi: 10.1042/bj1860217.
Abstract
- The effects of various concentrations of ascorbic acid on the quality and quantity of the insoluble extracellular matrices produced by two strains of cultured rat smooth-muscle cells were studied. 2. Ascorbic acid was necessary for the appearance of insoluble collagen in the extracellular matrix. 3. Secretion of soluble collagen continued in the absence of ascorbic acid, but this soluble collagen was markedly underhydroxylated. 4. The amount of insoluble collagen present in the matrix was directly related to the ascorbic acid concentration. 5. The insoluble collagen that appeared in the matrix under conditions where ascorbic acid was limiting was no more than 7% underhydroxylated. 6. In contrast, the amount of insoluble elastin produced was inversely proportional to the ascorbic acid concentration. 7. The elastin produced in the absence of ascorbic acid had the expected amino acid composition, but hydroxyproline was absent. 8. The hydroxyproline content of elastin was also directly dependent on the ascorbic acid concentration. 9. Ascorbic acid had variable effects on the quantity of glycoprotein(s) present in the matrix. 10. The appearance of insoluble collagen in the extracellular matrices produced by cultured human fibroblasts and calf endothelial cells was also completely dependent on the presence of ascorbic acid.
摘要
- 研究了不同浓度的抗坏血酸对两株培养的大鼠平滑肌细胞产生的不溶性细胞外基质的质量和数量的影响。2. 抗坏血酸是细胞外基质中出现不溶性胶原蛋白所必需的。3. 在没有抗坏血酸的情况下，可溶性胶原蛋白的分泌仍在继续，但这种可溶性胶原蛋白的羟基化程度明显不足。4. 基质中存在的不溶性胶原蛋白的量与抗坏血酸浓度直接相关。5. 在抗坏血酸有限的条件下出现在基质中的不溶性胶原蛋白的羟基化不足不超过7%。6. 相比之下，产生的不溶性弹性蛋白的量与抗坏血酸浓度成反比。7. 在没有抗坏血酸的情况下产生的弹性蛋白具有预期的氨基酸组成，但没有羟脯氨酸。8. 弹性蛋白的羟脯氨酸含量也直接取决于抗坏血酸浓度。9. 抗坏血酸对基质中存在的糖蛋白数量有不同的影响。10. 培养的人成纤维细胞和小牛内皮细胞产生的细胞外基质中不溶性胶原蛋白的出现也完全依赖于抗坏血酸的存在。
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