Ebling F, Hahn B H
Arthritis Rheum. 1980 Apr;23(4):392-403. doi: 10.1002/art.1780230402.
Sera from MRL/1, BXSB, and NZB/NZW mice, which develop IgG antibodies to DNA and glomerular deposits of DNA-antiDNA immune complexes, were studied by isoelectric focusing. A large array of IgG antibodies with isoelectric points ranging from pH 5.5--9.0 were found to bind double-stranded DNA. Antibodies with isoelectric points from 8.0--8.5 were significantly more frequent than antibodies focusing in all other pH ranges. In contrast, glomerular eluates from MRL/1 and NZB/NZW mice contained a restricted number of DNA-binding bands, all of which focused at pH 8.0--9.0. Anti-DNA with isoelectric points from pH 8.0--9.0 may be more pathogenic for the kidney than other subpopulations.
对MRL/1、BXSB和NZB/NZW小鼠的血清进行了等电聚焦研究，这些小鼠会产生针对DNA的IgG抗体以及DNA-抗DNA免疫复合物的肾小球沉积物。发现大量等电点范围在pH 5.5 - 9.0之间的IgG抗体能结合双链DNA。等电点在8.0 - 8.5之间的抗体比聚焦在所有其他pH范围内的抗体明显更常见。相比之下，MRL/1和NZB/NZW小鼠的肾小球洗脱液中含有的DNA结合带数量有限，所有这些带都聚焦在pH 8.0 - 9.0。等电点在pH 8.0 - 9.0之间的抗DNA对肾脏可能比其他亚群更具致病性。
