Hentati B, Ternynck T, Avrameas S, Payelle-Brogard B
Unité d'Immunocytochimie, URA 359 du CNRS, Institut Pasteur, Paris, France.
J Autoimmun. 1991 Apr;4(2):341-56. doi: 10.1016/0896-8411(91)90029-c.
Autoantibodies arising in (NZB x NZW)F1 (B/W) mice during the lupus-like syndrome were studied and compared to natural antibodies present in normal mice. The antibody activities were tested in sera, circulating immune complexes (CIC) and kidney eluates, using an enzyme immunoassay against a panel of self and non-self antigens: actin, myosin, tubulin, DNA, myoglobin, spectrin and trinitrophenylated bovine serum albumin (TNP/BSA). In the B/M mouse sera, IgM antibodies reacting with all the panel of antigens (PAg) and comparable to those of normal mice, increased moderately from 5 to 9 months and markedly during the last stage preceding death (10 months), when particularly high levels of anti-DNA, anti-tubulin and anti-myoglobin antibodies were noted. Polyreactive IgM antibodies present in CIC were moderately increased while those present in complexes deposited in kidneys were strongly enhanced after the 8th month. IgG antibodies showed an early increase (2 months) in B/W sera for anti-TNP activity, which remained more or less constant until death, while a later (5-6 months) and greater increase of activity, mainly directed against DNA but also against the other antigens of the panel, was observed. In CIC, IgG, mainly anti-DNA but also anti-TNP, were enhanced at the end of the disease while at the same time IgG reacting with all the PAg were found in kidney deposits. Isolation of antibodies from sera on a DNA-immunoadsorbent demonstrated that eluted IgM reacted with all the PAg but mainly with DNA, while IgG reactivity was more restricted to DNA and to a lesser degree to TNP. The D23 idiotype, characteristics of natural polyspecific antibodies, was expressed on IgM and IgG autoantibodies from B/W mice and was enhanced, particularly in kidneys, at the end of the disease. These results demonstrate that natural antibodies are a part of the population of increased autoantibodies in this disease and could participate with IgG anti-DNA antibodies in lupus.
研究了（新西兰黑鼠×新西兰白鼠）F1（B/W）小鼠在狼疮样综合征期间产生的自身抗体，并与正常小鼠体内存在的天然抗体进行了比较。使用酶免疫测定法针对一组自身和非自身抗原（肌动蛋白、肌球蛋白、微管蛋白、DNA、肌红蛋白、血影蛋白和三硝基苯基化牛血清白蛋白（TNP/BSA））检测血清、循环免疫复合物（CIC）和肾脏洗脱液中的抗体活性。在B/M小鼠血清中，与所有抗原组（PAg）反应且与正常小鼠相当的IgM抗体，在5至9个月时适度增加，在死亡前的最后阶段（10个月）显著增加，此时抗DNA、抗微管蛋白和抗肌红蛋白抗体水平特别高。CIC中存在的多反应性IgM抗体适度增加，而沉积在肾脏中的复合物中的IgM抗体在第8个月后强烈增强。B/W血清中的IgG抗体在抗TNP活性方面早期增加（2个月），直至死亡时或多或少保持恒定，而后期（5 - 6个月）活性增加更大，主要针对DNA，但也针对该组中的其他抗原。在CIC中，IgG主要是抗DNA但也抗TNP，在疾病末期增强，同时在肾脏沉积物中发现与所有PAg反应的IgG。从血清中在DNA免疫吸附剂上分离抗体表明，洗脱的IgM与所有PAg反应，但主要与DNA反应，而IgG反应性更局限于DNA，在较小程度上与TNP反应。天然多特异性抗体的特征性D23独特型在B/W小鼠的IgM和IgG自身抗体上表达，并在疾病末期增强，特别是在肾脏中。这些结果表明，天然抗体是该疾病中自身抗体增加群体的一部分，并且可能与IgG抗DNA抗体一起参与狼疮的发病。
