Malcolm S A, Hughes T C
Br J Dermatol. 1980 Mar;102(3):267-75. doi: 10.1111/j.1365-2133.1980.tb08139.x.
Using scanning electron microscopy it has been possible to demonstrate the location of bacteria on and within the stratum corneum of the human foot. Biopsies taken either by sectioning or by removing stratum corneum with cyanoacrylate ester adhesive were examined using a Jeol JSM-T20 scanning electron microscope. Bacteria could be seen easily on specimens from skin which had been occluded to increase the number of bacteria present. On the surface, bacteria were scattered widely in small colonies (usually containing less than ten bacteria). Although bacteria could be seen around the orifice of sweat ducts they did not preferentially favour these sites. Within the stratum corneum, bacteria could be found as relatively large colonies but these were usually associated with sweat ducts or the underside of the furrows in the skin surface. This study suggests that, in normal skin, bacteria are able to colonize both the surface and the depths of the stratum corneum.
利用扫描电子显微镜已能够证明细菌在人足部角质层上及角质层内的位置。通过切片或用氰基丙烯酸酯粘合剂去除角质层所取的活检样本，使用日本电子株式会社JSM-T20扫描电子显微镜进行检查。在已被封闭以增加细菌数量的皮肤标本上，细菌很容易被看到。在表面，细菌以小菌落的形式广泛散布（通常包含少于十个细菌）。尽管在汗腺导管口周围可以看到细菌，但它们并非优先聚集在这些部位。在角质层内，可以发现细菌形成相对较大的菌落，但这些菌落通常与汗腺导管或皮肤表面褶皱的底部相关。这项研究表明，在正常皮肤中，细菌能够在角质层的表面和深层定殖。
