Evans I H, Mathias A P
Biochim Biophys Acta. 1980 Apr 3;628(4):480-8. doi: 10.1016/0304-4165(80)90396-7.
Parenchymal cells, isolated from rat liver by a simple non-enzymic technique, were fractioned according to ploidy class by rate zonal centrifugation on sucrose density gradients. This method of fractionation applied to liver cells prelabelled in vivo with tritiated thymidine separated different size classes of cells synthesising DNA.
通过一种简单的非酶技术从大鼠肝脏中分离出的实质细胞,在蔗糖密度梯度上通过速率区带离心根据倍性类别进行分级分离。这种分级分离方法应用于体内预先用氚标记胸腺嘧啶核苷标记的肝细胞,可分离出合成DNA的不同大小类别的细胞。