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[肌酸激酶酶活性宏观波动光谱与蛋白质溶液pH值之间的关系]

[Relationship between the spectra of macroscopic fluctuations of creatine kinase enzyme activity and the pH of a protein solution].

作者信息

Shnol' S E, Britsina T Ia, Ivanova N P, Kolombet V A

出版信息

Biofizika. 1980 Mar-Apr;25(2):218-22.

PMID:7370334
Abstract

A canonic shape of the spectrum of macroscopic fluctuations of enzymic activity in creatinkinase solutions [1] is revealed within wide pH range. With pH decrease (from 10.0 to 6.0) the discreteness and reproducibility of the spectra decreases, at pH 5.0 no canonic spectrum was revealed. The discovered dependence of the spectrum shape on pH illustrates possibilities of spectral analysis of macroscopic fluctuations for characterization of the states of protein aqueous solutions.

摘要

在较宽的pH范围内揭示了肌酸激酶溶液中酶活性宏观波动光谱的典型形状。随着pH值降低(从10.0降至6.0),光谱的离散性和可重复性降低,在pH 5.0时未发现典型光谱。所发现的光谱形状对pH值的依赖性说明了通过宏观波动光谱分析来表征蛋白质水溶液状态的可能性。

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