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[宏观涨落——不同蛋白质和其他物质水溶液的一般特性。宏观涨落的统计光谱分析]

[Macroscopic fluctuations--a general property of aqueous solutions of different proteins and other substances. Statistical spectral analysis of macroscopic fluctuations].

作者信息

Shnol' S E, Kolombet V A, Ivanova N P, Britsina T I

出版信息

Biofizika. 1980 May-Jun;25(3):409-16.

PMID:7397255
Abstract

When studying enzymic activities in successively chosen portions of solutions of pyruvatekinase, hexokinase, lactatedehydrogenase, alkaline phosphatase, acetylcholinesterase and trypsin fast macroscopic fluctuations were revealed. These fluctuations were found earlier in protein preparations of the actomyosin complex and creatinkinase and described as "conformation fluctuations". Similar macroscopic fluctuations were also revealed when measuring the rate of reaction between ascorbic acid and dichlorphenolindophenol (DCAPI). The spectra of macroscopic fluctuations in solutions of different proteins and ascorbic acid+DCAPI are similar to each other in principle. This gives grounds to consider the ability towards macroscopic fluctuations to be a common property of solutions of different substances.

摘要

在对丙酮酸激酶、己糖激酶、乳酸脱氢酶、碱性磷酸酶、乙酰胆碱酯酶和胰蛋白酶溶液依次选取的部分进行酶活性研究时,发现了快速的宏观波动。这些波动 earlier 在肌动球蛋白复合物和肌酸激酶的蛋白质制剂中被发现,并被描述为“构象波动”。在测量抗坏血酸与二氯酚靛酚(DCAPI)之间的反应速率时,也发现了类似的宏观波动。不同蛋白质溶液以及抗坏血酸 + DCAPI 溶液中的宏观波动光谱原则上彼此相似。这使得有理由认为宏观波动能力是不同物质溶液的共同特性。 (注:原文中“earlier”翻译时感觉上下文不太通顺,可能存在信息不准确,你可再核对下原文。)

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