抗坏血酸在哺乳动物细胞培养中的诱变活性。
Mutagenic activity of ascorbate in mammalian cell cultures.
作者信息
Rosin M P, San R H, Stich H F
出版信息
Cancer Lett. 1980 Feb;8(4):299-305. doi: 10.1016/0304-3835(80)90145-7.
PMID:7370967
Abstract
Exposure of Chinese hamster ovary (CHO) cells to solutions of ascorbate (2--5 x 10(-4) M) resulted in the induction of somatic mutations at the hypoxanthineguanine phosphoribosyl transferase (HGPRT) locus. Mutant cells were resistant to 6-thioguanine (10 microgram/ml) and sensitive to HAT (hypoxanthine, aminopterin, thymidine) medium. Doses of ascorbate which were mutagenic were also toxic. Addition of catalase to such ascorbate concentrations prevented both mutagenesis and toxicity. This suggests that mutagenic metabolites of ascorbate may involve peroxide radicals.
摘要
将中国仓鼠卵巢（CHO）细胞暴露于抗坏血酸盐溶液（2 - 5×10⁻⁴ M）会导致次黄嘌呤鸟嘌呤磷酸核糖转移酶（HGPRT）位点发生体细胞突变。突变细胞对6-硫鸟嘌呤（10微克/毫升）具有抗性，对HAT（次黄嘌呤、氨基蝶呤、胸腺嘧啶）培养基敏感。具有致突变性的抗坏血酸盐剂量也是有毒的。向此类抗坏血酸盐浓度中添加过氧化氢酶可防止诱变和毒性。这表明抗坏血酸盐的诱变代谢产物可能涉及过氧化物自由基。
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