肝微粒体对N-亚硝基吗啉的脱亚硝化作用；细胞色素P-450的可能作用。

Denitrosation of N-nitrosomorpholine by liver microsomes; possible role of cytochrome P-450.

作者信息

Appel K E, Schrenk D, Schwarz M, Mahr B, Kunz W

出版信息

Cancer Lett. 1980 Mar;9(1):13-20. doi: 10.1016/0304-3835(80)90135-4.

Abstract

Liver microsomes from male mice and rats were incubated with N-nitrosomorpholine (MoNA) and an NADPH-regenerating system. The formation of nitrite was measured after induction or inhibition of the microsomal monooxygenase system. Pretreatment of the animals with phenobarbital (PB) enhanced nitrite formation by about 200%, while 3-methylcholanthrene (3-MC)-induction showed no exceptional effects. Various specific inhibitors of the monooxygenase function including carbon monoxide decreased nitrite formation. In conjunction with results obtained by spectra studies it is suggested that N-nitrosomorpholine is denitrosated by a reduction process in which cytochrome (cyt.) P-450 seems to be involved. Nitricoxide formed is partly converted to nitrite under these conditions.

摘要

将雄性小鼠和大鼠的肝脏微粒体与N-亚硝基吗啉（MoNA）及NADPH再生系统一起孵育。在诱导或抑制微粒体单加氧酶系统后，测定亚硝酸盐的生成量。用苯巴比妥（PB）对动物进行预处理可使亚硝酸盐生成量增加约200%，而3-甲基胆蒽（3-MC）诱导则未显示出特殊影响。包括一氧化碳在内的各种单加氧酶功能特异性抑制剂均可减少亚硝酸盐的生成。结合光谱研究结果表明，N-亚硝基吗啉通过一种还原过程进行脱亚硝基，其中细胞色素（cyt.）P-450似乎参与其中。在这些条件下，形成的一氧化氮部分转化为亚硝酸盐。