Floman Y, Eyre D R, Glimcher M J
Clin Orthop Relat Res. 1980 Mar-Apr(147):278-86.
Measurements of uptake in vivo of 3H-proline into 3H-hydroxyproline demonstrate that cartilage surfaces in rabbit knees with surgically induced osteoarthrosis (up to 3 months after operation) incorporated 2 to 5 times more activity into collagen than cartilage of control knees. The high uptake indicates that collagen synthesis was stimulated severalfold. In the lateral compartment of the experimental knee, which did not develop lesions, the 3H-proline metabolism was also stimulated, but this reaction subsided after 3 months. In the medial compartment, where the focal lesions developed, the stimulation continued. The newly made collagen was predominantly Type II; no evidence was found for a significant production of Type I collagen, even in the focal lesion on the medial tibial plateau more than a year after surgery. By radiochemical labelling with 3H-glucosamine,, an increase in the ratio of 3H-galactosamine to 3H-glucosamine was detected in all cartilage surfaces at intervals of 2-5 weeks after surgery. The increase was associated with the transient synovitis before lesions appeared, but not later. The observations suggest that synovitis and mechanical stresses are both important in inducing osteoarthrotic lesions.
对兔膝关节手术诱导骨关节炎（术后长达3个月）中3H - 脯氨酸向3H - 羟脯氨酸的体内摄取量测量表明，与对照膝关节软骨相比，手术诱导骨关节炎的兔膝关节软骨表面在胶原蛋白中掺入的活性高2至5倍。高摄取量表明胶原蛋白合成被刺激了几倍。在未出现病变的实验膝关节外侧间室，3H - 脯氨酸代谢也受到刺激，但这种反应在3个月后消退。在出现局灶性病变的内侧间室，刺激持续存在。新合成的胶原蛋白主要是II型；即使在手术后一年多的内侧胫骨平台局灶性病变处，也未发现有大量I型胶原蛋白产生的证据。通过用3H - 葡糖胺进行放射化学标记，在术后2 - 5周的间隔时间内，在所有软骨表面均检测到3H - 半乳糖胺与3H - 葡糖胺的比例增加。这种增加与病变出现前的短暂滑膜炎有关，但在病变出现后则无关。这些观察结果表明，滑膜炎和机械应力在诱导骨关节炎病变中都很重要。
