Solano A R, Garcia-Vela A, Catt K J, Dufau M L
Endocrinology. 1980 Jun;106(6):1941-8. doi: 10.1210/endo-106-6-1941.
The biological and immunological activities of serum and pituitary LH were analyzed in normal and castrated rats using the rat interstitial cell testosterone assay and RIAs for intact rat LH (rLH), rLH alpha, and rLH beta. After castration, the bioactivity to immunoactivity ratio of both serum and pituitary LH decreased by about 50% between the 5th and 25th days and returned to normal by 60 days. This decrease in the bioactivity to immunoreactivity ratio of LH in castrated males was not due to increased subunit production and was prevented by the administration of testosterone propionate (50 micrograms/day) from the time of castration. These observations indicate that androgens of their metabolites modify the bioactivity of the circulating and stored forms of the LH molecule.
利用大鼠间质细胞睾酮测定法以及针对完整大鼠促黄体生成素（rLH）、rLHα和rLHβ的放射免疫分析法（RIA），对正常和去势大鼠血清及垂体促黄体生成素的生物学和免疫学活性进行了分析。去势后，血清和垂体促黄体生成素的生物活性与免疫活性之比在第5天至第25天之间下降了约50%，并在60天时恢复正常。去势雄性大鼠促黄体生成素生物活性与免疫反应性之比的这种下降并非由于亚基产量增加，且从去势时起给予丙酸睾酮（50微克/天）可防止这种情况发生。这些观察结果表明，雄激素及其代谢产物会改变促黄体生成素分子循环和储存形式的生物活性。
