Ittrich G
Endokrinologie. 1980 Jan;75(1):13-9.
A simple technique for direct quantitative evaluation of hemagglutination or hemagglutination inhibition tests used in immunologic proteo-hormone assay systems is described. The test is performed in calibrated glass capillaries (internal diameter 2.5...4 mm) at horizontal position. After sedimentation the non-agglutinated erythrocytes form two fine parallel stripes along the bottom of the capillaries. The distance between the stripes depends on the amount of additional free antigen brought into the system. It can be measured by the slit of a micrometer screw or a corresponding equipped microscope and might be used as y-variable for drawing calibration curves. At well standardized test conditions and for selected concentration ranges coefficients of variation between 10 and 15% can be achieved. So quantitative determinations can be made without serial sample dilutions to be prepared, covering concentrations ranges between 1 to 10.
本文描述了一种用于免疫蛋白激素分析系统中血凝或血凝抑制试验直接定量评估的简单技术。该试验在水平放置的校准玻璃毛细管（内径2.5……4毫米）中进行。沉降后，未凝集的红细胞沿毛细管底部形成两条细的平行条纹。条纹之间的距离取决于引入系统中的额外游离抗原的量。它可以通过测微螺杆的狭缝或相应配备的显微镜进行测量，并可用作绘制校准曲线的y变量。在标准化良好的试验条件下，对于选定的浓度范围，变异系数可达到10%至15%。因此，无需制备系列样品稀释液即可进行定量测定，浓度范围为1至10。
