通过小角X射线散射对溶液中细螯龙虾血蓝蛋白24-S成分的四级结构进行的研究。

Studies by small-angle X-ray scattering of the quaternary structure of the 24-S component of the haemocyanin of Astacus leptodactylus in solution.

作者信息

Pilz I, Goral K, Hoylaerts M, Witters R, Lontie R

出版信息

Eur J Biochem. 1980 Apr;105(3):539-43. doi: 10.1111/j.1432-1033.1980.tb04530.x.

DOI:10.1111/j.1432-1033.1980.tb04530.x

Abstract

The haemocyanin of Astacus leptodactylus was studied in several buffers at three pH values. The best stability and lowest mean deviation on repeating the measurements were found at pH 7.2 in a Tris-HCl buffer. The following molecular parameters were determined: radius of gyration 6.90 nm, radius of gyration of the cross-section 3.87 nm, maximum dimension 21.5 nm, relative molecular mass 854000, volume 1440 nm3, hydration 0.27 g H2O/g protein. The theoretical scattering curves of a large number of models were calculated to find one fitting these data and the experimental scattering curve. The model with the best agreement was compared with an electron micrograph.

摘要

在三种pH值下，于几种缓冲液中对细指螯虾的血蓝蛋白进行了研究。在Tris-HCl缓冲液中pH 7.2时，发现重复测量时稳定性最佳且平均偏差最低。测定了以下分子参数：回转半径6.90 nm、横截面回转半径3.87 nm、最大尺寸21.5 nm、相对分子质量854000、体积1440 nm³、水合度0.27 g H₂O/g蛋白质。计算了大量模型的理论散射曲线，以找到一个与这些数据和实验散射曲线拟合的模型。将一致性最佳的模型与电子显微镜照片进行了比较。