关于苹果螺β-血蓝蛋白四级结构的小角X射线散射研究。

Studies by small-angle X-ray scattering of the quaternary structure of the beta-haemocyanin of Helix pomatia.

作者信息

Berger J, Pilz I, Witters R, Lontie R

出版信息

Eur J Biochem. 1977 Oct 17;80(1):79-82. doi: 10.1111/j.1432-1033.1977.tb11858.x.

DOI:10.1111/j.1432-1033.1977.tb11858.x

Abstract

Helix pomatia beta-haemocyanin was studied in solution by small-angle X-ray scattering. The following molecular parameters were determined: molecular weight = 9.02 X 10(6), volume = 14000 nm3, radius of gyration = 18.4 nm, radius of the spherical subunits = 2.5 +/- 0.2 nm. With these data, and with information of dissociation products described in a former paper, a model of the molecule was built whose theoretical scattering curve showed good agreement with the experimental one. The model consists of 160 spherical subunits of a radius of 2.5 nm; 12 rings each built up of 10 spheres form the outer wall of a hollow cylinder; 20 subunits are situated at the inner side of each end.

摘要

利用小角X射线散射对玛瑙螺β-血蓝蛋白在溶液中的情况进行了研究。测定了以下分子参数：分子量 = 9.02×10⁶，体积 = 14000 nm³，回转半径 = 18.4 nm，球形亚基半径 = 2.5±0.2 nm。根据这些数据以及前文所述解离产物的信息，构建了一个分子模型，其理论散射曲线与实验曲线吻合良好。该模型由160个半径为2.5 nm的球形亚基组成；12个环，每个环由10个球体构成，形成一个空心圆柱体的外壁；每个端部内侧有20个亚基。