Cook H W
Biochim Biophys Acta. 1978 Dec 22;531(3):245-56. doi: 10.1016/0005-2760(78)90206-0.
Mixtures of [1-14C]elaidic acid and [9,10-3H]oleic acid, as well as [1-14C]elaidic or [1-14C]oleic acid alone, were administered by intracerebral injection to 10-day old and adult rats to examine the relative incorporation and metabolism of trans- and cis-monounsaturated fatty acids in developing and mature brain. Rates and extents of total incorporation of label from trans- and cis-acid into complex lipids were similar. Maximum labeling of the neutral lipid, mainly triacylglycerol and cholesterol, occurred prior to 4 h after injection whereas phospholipid, mainly choline phosphoglyceride, was maximally labeled at 4--8 h after injection. The decrease in labeled phospholipid from 24 to 90 h was greater with elaidate than with oleate. At 8 h labeled elaidic acid was preferentially esterified in the 1-position of all phosphoglycerides whereas labeled oleic acid, in contrast to the positional distribution of endogenous oleate, was randomly distributed. Label from elaidate found in palmitate increased with time to 26% of the total recovered label by 48 h. Thus, although some of the trans-fatty acid is oxidized and its labeled carbon is redistributed, most is incorporated unaltered into complex lipid of brain at rates similar to those for its cis-isomer. The developing central nervous system, therefore, does not metabolically exclude the trans-acid, elaidic acid, from membrane lipids.
将[1-¹⁴C]反油酸和[9,10-³H]油酸的混合物，以及单独的[1-¹⁴C]反油酸或[1-¹⁴C]油酸，通过脑内注射给予10日龄和成年大鼠，以研究发育中和成熟大脑中反式和顺式单不饱和脂肪酸的相对掺入和代谢情况。反式和顺式酸中标记物掺入复合脂质的总速率和程度相似。中性脂质（主要是三酰甘油和胆固醇）的最大标记出现在注射后4小时之前，而磷脂（主要是胆碱磷酸甘油酯）在注射后4至8小时达到最大标记。与油酸相比，反油酸处理后，标记磷脂在24至90小时内的减少幅度更大。在8小时时，标记的反油酸优先酯化在所有磷酸甘油酯的1位，而标记的油酸与内源性油酸的位置分布不同，是随机分布的。在棕榈酸中发现的反油酸标记物随时间增加，到48小时时达到回收总标记物的26%。因此，尽管一些反式脂肪酸被氧化，其标记的碳被重新分布，但大多数以与顺式异构体相似的速率完整地掺入大脑的复合脂质中。因此，发育中的中枢神经系统在代谢上并不排除反式酸（反油酸）进入膜脂质。
