Kurinenko B M, Kalacheva N V, Penzikova G A, Skokova I F
Biokhimiia. 1978 Nov;43(11):1994-2001.
Preparations of pancreatic RNAase, ribonuclease Act. rimosus and nuclease Ser. marcescens covalently bound to water-soluble derivatives of polysaccharides (m-aminobenzyl-oxymethyl ether of dextran and mannan, 4beta-oxyethylsulphonylanisol, 4beta-oxyethylsulphonylaniline, 3-Cl-2-oxypropyl ethers of dextran and dialdehydedextran) have been obtained. The yields and thermal stability of immobilized nucleases depend both upon the amount and nature of the functional groups which activate the polysaccharide. Polysaccharide aminoaryl ethers capable of binding to proteins by azocoupling present special interest in view of their utilization as modifying baskings. Regulation of effectiveness of the azocoupling reaction by means of structural changes of the diazocomponent and the reaction conditions were shown. All this allows to obtain immobilized enzymes with different physico-chemical properties.
