Kurinenko B M, Beliaeva M I, Cherepneva I E, Viesture Z A
Vopr Onkol. 1977;23(11):94-8.
The antitumor effect of Ser. marcescens nuclease, modified by covalent binding using the method of diazocoupling with m-aminobenzyloxymethyl dextran of molecular weight 20 000, 40 000, 60 000, exceeds 3--4 times the antitumor effect of the native enzyme in relation to Ehrlich ascites carcinoma in contact tumor cells exposure. In intramuscular injection of the enzyme the nuclease preparation modified by dextran of molecular weight 40 000 showed the greatest antitumor activity against Ehrlich carcinoma and sarcoma 37.
用重氮偶联法将分子量为20000、40000、60000的间氨基苄氧基甲基葡聚糖与粘质沙雷氏菌核酸酶共价结合进行修饰，在接触肿瘤细胞时，其对艾氏腹水癌的抗肿瘤作用比天然酶的抗肿瘤作用高出3至4倍。在肌肉注射该酶时，分子量为40000的葡聚糖修饰的核酸酶制剂对艾氏癌和肉瘤37显示出最大的抗肿瘤活性。
