Geneix A, Lécher P, Jaffray J Y, Lagarde N, Hermabessière J
Hum Genet. 1980;53(3):327-33. doi: 10.1007/BF00287051.
Human meiotic chromosomes, from spermatocytes and ovocytes, are described after observations of whole mount preparations under E.M. Small testicular and ovarian fragments are put in distillated water, then macerated; the cell suspension is spread on the surface of sheet copper grids covered with formvar plus collodion films. After dehydratation interesting stages are selected under L.M. before observations under E.M. Zygotene and pachytene are the most common stages. During pachytene the chromomeres are well individualized; the synaptonemal complex may be observed; chromatin fibers connect the chromosomes to nuclear pores, interchromosomal fibers joint the bivalents. Zygotene and pachytene bivalents are very similar in the male and the feminine germ cells.
通过在电子显微镜下观察整装标本,对来自精母细胞和卵母细胞的人类减数分裂染色体进行了描述。将小块睾丸和卵巢组织置于蒸馏水中,然后进行浸解;细胞悬液铺展在覆盖有福尔马弗和火棉胶膜的铜网表面。脱水后,在光学显微镜下选择感兴趣的阶段,然后再在电子显微镜下观察。偶线期和粗线期是最常见的阶段。在粗线期,染色粒清晰可辨;可以观察到联会复合体;染色质纤维将染色体连接到核孔,染色体间纤维连接二价体。偶线期和粗线期的二价体在雄性和雌性生殖细胞中非常相似。