Heinz F X, Kunz C
Intervirology. 1980;13(3):169-77. doi: 10.1159/000149122.
Crosslinking of tick-borne encephalitis virus with dimethylsuberimidate followed by SDS-PAGE analysis yielded polymers of the core protein V2 and the viral glycoprotein V3, both in continuously decreasing amounts. As the two structural entities of flaviviruses - cores and viral envelope - are apparently crosslinked independently from one another, we employed this property to study the action of different detergents at various concentrations on either the viral envelope or core. Triton X-100 and octylglucoside had no influence on the core but did dissociate the envelope into a V3-dimer. Centrifugation in density gradients containing these detergents yielded a 5-6S lipid-free hemagglutinating subunit which most probably represents a V3-dimer. In the presence of Triton X-100 this complex contains V1 in addition to V3.
用辛二酸二甲酯使蜱传脑炎病毒交联，随后进行SDS-PAGE分析，结果显示核心蛋白V2和病毒糖蛋白V3均形成聚合物，且二者含量均持续减少。由于黄病毒的两个结构实体——核心和病毒包膜——显然是彼此独立交联的，我们利用这一特性研究了不同浓度的不同去污剂对病毒包膜或核心的作用。Triton X-100和辛基葡糖苷对核心无影响，但能使包膜解离成V3二聚体。在含有这些去污剂的密度梯度中离心，得到一个5-6S的无脂血凝亚基，它很可能代表一个V3二聚体。在Triton X-100存在的情况下，该复合物除了含有V3外还含有V1。
