一种用于电生理测量的多功能高压腔室。

A versatile high-pressure chamber for electrophysiological measurements.

作者信息

Parmentier J L, Shrivastav B B, Bennett P B

出版信息

J Appl Physiol Respir Environ Exerc Physiol. 1980 Mar;48(3):562-6. doi: 10.1152/jappl.1980.48.3.562.

DOI:10.1152/jappl.1980.48.3.562

Abstract

A modular high-pressure chamber is described. This chamber will allow stable microelectrode recordings to be made for a variety of intracellular preparations at pressure of 200-300 atmospheres absolute. Its features include internal temperature control, easy visibility, continuous perfusion, electrical penetrations, and manipulation of several internal controls while the system is at pressure. The small size and high versatility of the Wilson chamber make it a convenient and inexpensive research unit for experiments in the moderately high-pressure ranges that affect biological preparations.

摘要

本文描述了一种模块化高压舱。该高压舱能够在200至300个绝对大气压的压力下，对多种细胞内制剂进行稳定的微电极记录。其特点包括内部温度控制、可视性良好、连续灌注、电穿透以及在系统处于压力状态时对多个内部控件的操作。威尔逊高压舱体积小、通用性强，使其成为在影响生物制剂的中等高压范围内进行实验的便捷且经济的研究设备。