Brown J P, Wright P W, Hart C E, Woodbury R G, Hellström K E, Hellström I
J Biol Chem. 1980 Jun 10;255(11):4980-3.
Spleen cells from a mouse immunized with human melanoma cells were fused with mouse myeloma cells, and somatic cell hybrids were grown in selective medium. Eight hybrids, which secreted antibodies to protein antigens of the melanoma cell line, were identified by immunoprecipitation of a 125I-labeled melanoma cell lysate followed by sodium dodecyl sulfate-gel electrophoresis of the immunoprecipitates and autoradiography. Seven of the eight melanoma proteins identified in this way were present at the cell surface. Two of the cell surface proteins, p80 and p97, were not detected in autologous fibroblasts.
用人类黑色素瘤细胞免疫的小鼠的脾细胞与小鼠骨髓瘤细胞融合，体细胞杂种在选择培养基中培养。通过对125I标记的黑色素瘤细胞裂解物进行免疫沉淀，随后对免疫沉淀物进行十二烷基硫酸钠-凝胶电泳和放射自显影，鉴定出8个分泌针对黑色素瘤细胞系蛋白质抗原抗体的杂种。通过这种方式鉴定出的8种黑色素瘤蛋白中有7种存在于细胞表面。两种细胞表面蛋白p80和p97在自体成纤维细胞中未检测到。
