气相色谱法测定人血浆和尿液中低浓度苯甲酸

Gas chromatographic determination of low concentrations of benzoic acid in human plasma and urine.

作者信息

Sioufi A, Pommier F

出版信息

J Chromatogr. 1980 Feb 8;181(2):161-8. doi: 10.1016/s0378-4347(00)81601-1.

Abstract

A method for the determination of benzoic acid down to concentrations of 10 ng/ml in plasma or urine is described. After addition of an internal standard, benzoic acid is extracted at acid pH into diethyl ether. Both compounds are derivatized with pentafluorobenzyl bromide. The derivatives are determined by gas chromatography using a 63Ni electron-capture detector. Hippuric acid is hydrolysed in plasma and urine and total benzoic acid is determined by the same technique.

摘要

本文描述了一种测定血浆或尿液中低至10 ng/ml苯甲酸浓度的方法。加入内标后，在酸性pH条件下将苯甲酸萃取到乙醚中。两种化合物均用五氟苄基溴进行衍生化。衍生物通过使用63Ni电子捕获检测器的气相色谱法进行测定。马尿酸在血浆和尿液中水解，总苯甲酸通过相同技术测定。