Geleff S, Böck P
Histochemistry. 1984;81(6):543-9. doi: 10.1007/BF00489533.
Complex carbohydrate components of secretory granules and the glycocalix were analysed in surface epithelia, endoepithelial glands and exoepithelial tubuloalveolar glands of the biliary-ductular system (guinea pig). Brunner glands and pyloric glands were studied for comparison. The columnar epithelial cells of the gallbladder and biliary ducts displayed a well-developed PAS-positive apical glycocalix. These materials strongly bound Ricinus communis A I, Ulex europaeus I, Lotus tetragonolobus A and wheat-germ-A lectins. With the exception of Lotus A lectin which did not bind at all, the same lectins stained the basolateral cell surface. The secretory granules in the supranuclear regions of surface epithelia and in the exoepithelial glands strongly bound Ricinus A I, Ulex europaeus I, wheat-germ-A and Helix pomatia lectins. Concanavalin A was less intensively bound by the secretions of tubuloalveolar glands than by the secretory granules in surface epithelia. The luminal and basolateral cell surfaces of glandular cells in the exoepithelial glands were stained by the same spectrum of lectins as were the columnar cells of surface epithelia, but the staining was less distinct. In the guinea pig, the lectin-binding patterns of tubuloalveolar glands in the biliary ducts closely resembled those of Brunner glands and pyloric glands. The secretions of the tubuloalveolar glands were different from the secretion of surface epithelia, as they bound Concanavalin A less intensively.
对胆管系统（豚鼠）的表面上皮、内上皮腺和外上皮管泡状腺中分泌颗粒和糖萼的复合碳水化合物成分进行了分析。为作比较，研究了布伦纳腺和幽门腺。胆囊和胆管的柱状上皮细胞显示出发育良好的PAS阳性顶端糖萼。这些物质与蓖麻凝集素A I、荆豆凝集素I、百脉根凝集素A和麦胚凝集素A强烈结合。除完全不结合的百脉根A凝集素外，相同的凝集素对基底外侧细胞表面进行了染色。表面上皮细胞核上区和外上皮腺中的分泌颗粒与蓖麻凝集素A I、荆豆凝集素I、麦胚凝集素A和蜗牛凝集素强烈结合。刀豆球蛋白A与管泡状腺分泌物的结合强度低于与表面上皮分泌颗粒的结合强度。外上皮腺腺细胞的管腔和基底外侧细胞表面被与表面上皮柱状细胞相同谱的凝集素染色，但染色不太明显。在豚鼠中，胆管管泡状腺的凝集素结合模式与布伦纳腺和幽门腺的非常相似。管泡状腺的分泌物与表面上皮的分泌物不同，因为它们与刀豆球蛋白A的结合强度较低。
