Staack H, Davidson B, Schulz H
Lipids. 1980 Mar;15(3):175-8. doi: 10.1007/BF02540965.
Antibodies to pig heart 3-ketoacyl-CoA thiolase inhibited almost completely and in a parallel fashion thiolase and the acetyl-CoA-dependent fatty acid elongation system present in an acetone powder extract of pig heart mitochondria. This finding leads to the conclusion that mitochondrial fatty acid elongation occurs by reversal of fatty acid oxidation. Several lines of evidence point to the thiolase-catalyzed condensation reaction as the rate-limiting step in the formation of elongated products. However, the accumulation of hydroxy acids suggests the enoyl-CoA reductase activity is limiting in the synthesis of saturated fatty acids.
针对猪心脏3-酮酰基辅酶A硫解酶的抗体几乎完全抑制了猪心脏线粒体丙酮粉提取物中存在的硫解酶以及乙酰辅酶A依赖性脂肪酸延长系统，且抑制方式呈平行关系。这一发现得出如下结论：线粒体脂肪酸延长是通过脂肪酸氧化的逆向反应发生的。多条证据表明，硫解酶催化的缩合反应是延长产物形成过程中的限速步骤。然而，羟基酸的积累表明，烯酰辅酶A还原酶活性在饱和脂肪酸合成中是受限的。
