弗瑞德白血病细胞分化的化学诱导剂可抑制淋巴细胞有丝分裂。

Chemical inducers of differentiation in Friend leukaemia cells inhibit lymphocyte mitogenesis.

作者信息

Stenzel K H, Schwartz R, Rubin A L, Novogrodsky A

出版信息

Nature. 1980 May 8;285(5760):106-8. doi: 10.1038/285106a0.

Abstract

Several phorbol esters, the potent tumour-promoting agents isolated from croton oil, induce proliferation of human lymphocytes and enhance the mitogenic effect of lectins on bovine lymphocytes. While studying the mitogenic properties of one of these agents, phorbol myristate acetate (PMA), we found that dimethyl sulphoxide (DMSO), frequently used as a solvent for PMA, markedly inhibits PMA-induced mitogenesis at DMSO concentrations that have little effect on phytohaemagglutinin (PHA)-induced responses. DMSO, as well as a variety of other organic compounds, induce erythroid differentiation in Friend leukaemia (FL) cells. Phorbol esters, on the other hand, are potent inhibitors of both spontaneous and induced cellular differentiation. We therefore investigated the relationship between the potency of compounds to induce erythroid differentiation in FL cells and their potency to inhibit lymphocyte proliferation induced by PMA and other mitogens. We report here that many of the compounds that induce erythroid differentiation in FL cells are similar to DMSO in selectively suppressing PMA-induced lymphocyte mitogenesis.

摘要

几种佛波酯是从巴豆油中分离出的强效肿瘤促进剂，可诱导人淋巴细胞增殖，并增强凝集素对牛淋巴细胞的促有丝分裂作用。在研究其中一种试剂佛波醇肉豆蔻酸酯乙酸酯（PMA）的促有丝分裂特性时，我们发现，常用作PMA溶剂的二甲基亚砜（DMSO），在对植物血凝素（PHA）诱导的反应影响很小的DMSO浓度下，能显著抑制PMA诱导的有丝分裂。DMSO以及其他多种有机化合物可诱导弗瑞德白血病（FL）细胞发生红系分化。另一方面，佛波酯是自发和诱导细胞分化的强效抑制剂。因此，我们研究了化合物在FL细胞中诱导红系分化的能力与其抑制PMA和其他有丝分裂原诱导的淋巴细胞增殖的能力之间的关系。我们在此报告，许多在FL细胞中诱导红系分化的化合物在选择性抑制PMA诱导的淋巴细胞有丝分裂方面与DMSO相似。