高比活度核苷-γ-[32P]三磷酸的化学合成。
Chemical synthesis of nucleoside-gamma-[32P]triphosphates of high specific activity.
作者信息
Janecka A, Panusz H, Pankowski J, Koziołkiewicz W
出版信息
Prep Biochem. 1980;10(1):27-35. doi: 10.1080/00327488008061716.
PMID:7375446
Abstract
A simple chemical procedure for the preparation of four common ribonucleoside 5-gamma-[32P]triphosphates of high specific activity (up to 10 Ci/mmole) based on the condensation of orthophosphoric acid with the corresponding nucleoside 5-diphosphate in the presence of ethyl chloroformate as well as the methods of purification and identification of the products are described.
摘要
描述了一种简单的化学方法，用于制备四种具有高比活度（高达10居里/毫摩尔）的常见核糖核苷5-γ-[32P]三磷酸，该方法基于在氯甲酸乙酯存在下正磷酸与相应的核苷5-二磷酸的缩合反应，以及产物的纯化和鉴定方法。
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