一种通过化学合成快速制备腺苷 5'-γ-[32P]三磷酸的方法。

A method of the rapid preparation of adenosine 5'-gamma-[32P] triphosphate by chemical synthesis.

作者信息

Koziołkiewicz W, Pankowski J, Janecka A

出版信息

Prep Biochem. 1978;8(6):471-8. doi: 10.1080/00327487808061664.

PMID:219425

Abstract

A new chemical method for the synthesis of adenosine 5'-gamma-[32P] triphosphate has been developed based on the reaction of adenosine 5'-diphosphate with ethyl chloroformate. The resulting active mixed anhydride was able to react with [32P]-triethylammonium orthophosphate to give gamma-[32P]ATP.

摘要

基于腺苷5'-二磷酸与氯甲酸乙酯的反应，已开发出一种合成腺苷5'-γ-[32P]三磷酸的新化学方法。所得的活性混合酸酐能够与[32P] - 正磷酸三乙铵反应生成γ-[32P]ATP。