Roland B, Pallotta D
Can J Biochem. 1978 Nov;56(11):1021-7. doi: 10.1139/o78-161.
The rye and calf histones were fractionated by differential solubility and exclusion chromatography. Antibodies were produced against histones H1, H2A, H2B, and H3 of both species and the extent of cross-reaction was measured by microcomplement fixation. The results allowed the identification of rye histones H2A and H2B. The near structural identity of the H3 histones was confirmed by the small immunological distance which indicated an amino acid difference of less than 2% between rye and calf. The interspecific differences found for both H2A and H2B were greater, the amino acid difference estimated from the immunological distance being between 10 and 20%. H1 gave no immunological cross-reaction, indicating a large (greater than 40%) difference in the structure of this protein in the two species. The extent of variation that a histone fraction shows between plant and animal species is probably related to its role in chromatin organization.
通过差异溶解度和排阻色谱法对黑麦和小牛的组蛋白进行分级分离。制备了针对这两个物种的组蛋白H1、H2A、H2B和H3的抗体，并通过微量补体结合法测定交叉反应程度。结果确定了黑麦组蛋白H2A和H2B。H3组蛋白近乎相同的结构通过微小的免疫距离得到证实，这表明黑麦和小牛之间的氨基酸差异小于2%。H2A和H2B的种间差异更大，根据免疫距离估计的氨基酸差异在10%至20%之间。H1没有免疫交叉反应，表明这两个物种中该蛋白的结构存在很大差异（大于40%）。组蛋白组分在植物和动物物种之间表现出的变异程度可能与其在染色质组织中的作用有关。
