Bustin M, Reeder R H, McKnight S L
J Biol Chem. 1977 May 10;252(9):3099-101.
Antibodies elicited by purified calf thymus histone fractions are used to quantitate the immunological cross-reactions between calf and Drosophila histones. The index of dissimilarity between calf and Drosophila histones are: H4, 1.0; H2B, 1.0; H3, 1.6; H2A, 2.0; H1, 16. These values respectively correspond to 0, 0, 4, 6, and 27% difference in amino acid composition. The differences in amino acid composition assessed by immunological techniques are in agreement with values obtained by amino acid analysis (Alfageme, C. R., Zweidler, A., Mahowald, A., and Cohen, L. H. (1974) J Biol. Chem. 249, 3729-3736 and Oliver, D., and Chalkley, R. (1972) Exp. Cell. Res. 73, 295-302). The strong immunological cross-reaction between calf and Drosophila histones H4, H3, H2A, and H2B leads us to suggest that antibodies elicited against purified histone fractions derived from calf thymus can serve as "universal" reagents for various studies of chromatin-bound histones. The use of standard preparations of antisera will facilitate comparisons of results obtained in different laboratories with various experimental systems.
由纯化的小牛胸腺组蛋白组分引发的抗体用于定量小牛和果蝇组蛋白之间的免疫交叉反应。小牛和果蝇组蛋白之间的差异指数为：H4，1.0；H2B，1.0；H3，1.6；H2A，2.0；H1，16。这些值分别对应氨基酸组成上0%、0%、4%、6%和27%的差异。通过免疫技术评估的氨基酸组成差异与通过氨基酸分析获得的值一致（Alfageme, C. R., Zweidler, A., Mahowald, A., and Cohen, L. H. (1974) J Biol. Chem. 249, 3729 - 3736以及Oliver, D., and Chalkley, R. (1972) Exp. Cell. Res. 73, 295 - 302）。小牛和果蝇组蛋白H4、H3、H2A和H2B之间强烈的免疫交叉反应使我们认为，针对从小牛胸腺衍生的纯化组蛋白组分引发的抗体可作为用于染色质结合组蛋白各种研究的“通用”试剂。使用抗血清的标准制剂将便于比较在不同实验室使用各种实验系统获得的结果。
