Magnus E M
Scand J Haematol. 1980 Mar;24(3):243-53. doi: 10.1111/j.1600-0609.1980.tb01333.x.
In response to specific treatment of vitamin B12 deficient, anaemic patients there is an influx of folate into the young, circulating red cells. To separate new and old cells, capillary tubes filled with whole blood were centrifuged and the packed red cell column divided into top (T), middle (M) and bottom (B) layer. The newest cells are found in the T layer. The increase in red cell folate (RCF) concentration starts before, during or after the reticulocyte response, and is therefore not directly related to folate metabolism in the red precursor cells in the marrow. The low RCF concentration at the peak of the reticulocyte response in some of the cases demonstrates that the folate material, which may have been accumulated in the red precursor cells in the marrow, may be lost by the time the red cells enter the peripheral blood. The influx of folate into the young, circulating red cells is rapidly followed by an efflux of folate, suggesting that much of the folate material is still in the monoglutamate form. A new influx of folate is noted after a time lapse of from 5 to 10 d. Iron deficiency seems to prevent the uptake of folate by the circulating red cells.
针对维生素B12缺乏性贫血患者的特定治疗，叶酸会流入年轻的循环红细胞中。为了分离新老细胞，将装有全血的毛细管进行离心，使压紧的红细胞柱分为顶部（T）、中部（M）和底部（B）层。最新的细胞位于T层。红细胞叶酸（RCF）浓度的增加在网织红细胞反应之前、期间或之后开始，因此与骨髓中红细胞前体细胞的叶酸代谢没有直接关系。在某些病例中，网织红细胞反应峰值时RCF浓度较低，这表明可能已在骨髓红细胞前体细胞中积累的叶酸物质，在红细胞进入外周血时可能已经流失。叶酸流入年轻的循环红细胞后，很快就会有叶酸流出，这表明大部分叶酸物质仍以单谷氨酸盐形式存在。经过5至10天的时间间隔后，会出现新的叶酸流入。缺铁似乎会阻止循环红细胞对叶酸的摄取。
