Johnson M K, Clothier B
Toxicol Lett. 1980 Jan;5(1):95-8. doi: 10.1016/0378-4274(80)90154-x.
Chymotrypsin and neurotoxic esterase (NTE) have some similarities. After inhibition of concentrated (80-800 micro M) chymotrypsin by aryl saligenin cyclic phosphates it is known that aging occurs and some phenolic material becomes attached to protein. This binding has now been shown to be a manifestation of non-specific reaction with any available electrophile such as Tris, reduced glutathione (GSH), or protein. The reaction is therefore not a model for the 100% efficient transfer of alkyl groups to protein which occurs during aging of NTE inhibited by dialkyl phosphates.
胰凝乳蛋白酶和神经毒性酯酶（NTE）有一些相似之处。已知在芳基水杨苷环磷酸酯对浓缩的（80 - 800微摩尔）胰凝乳蛋白酶进行抑制后，会发生老化现象，并且一些酚类物质会附着到蛋白质上。现在已经表明，这种结合是与任何可用的亲电试剂（如Tris、还原型谷胱甘肽（GSH）或蛋白质）发生非特异性反应的表现。因此，该反应不是二烷基磷酸酯抑制NTE老化过程中烷基100%有效转移至蛋白质的模型。
