Funder J
Acta Physiol Scand. 1980 Jan;108(1):31-7. doi: 10.1111/j.1748-1716.1980.tb06497.x.
This work deals with the possibility that cations may be transported through the human red blood cell membrane as negatively charged ion pairs of the type XCO3-. Passive sodium influx into human erythrocytes was increased 4-fold when 150 mM chloride was substituted with bicarbonate in the medium. This increased permeability to sodium was completely abolished when the cells were pretreated with a specific inhibitor of anion transport (DIDS). Both bicarbonate-induced sodium influx and chloride exchange were linearly related to DIDS-binding. Both transport processes were completely inhibited by the binding of 1.1 X 10(6) DIDS molecules per cell. The presence of bicarbonate and/or pretreatment of the cells with DIDS did not change the permeability of the erythrocyte membrane to K+, Rb+, and Cs+ to any significant extent. The experimental findings support the hypothesis that Li+ and Na+, but not K+, Rb+, and Cs+ form monovalent negatively charged ion pairs with CO3--, which traverse the membrane through the anion exchange system.
本研究探讨了阳离子可能以XCO3- 这种带负电荷的离子对形式通过人红细胞膜进行转运的可能性。当培养基中的150 mM氯离子被碳酸氢盐替代时，人红细胞被动钠内流增加了4倍。当细胞用阴离子转运的特异性抑制剂（DIDS）预处理时，这种增加的钠通透性完全被消除。碳酸氢盐诱导的钠内流和氯交换都与DIDS结合呈线性相关。每细胞结合1.1×10(6)个DIDS分子时，这两种转运过程都被完全抑制。碳酸氢盐的存在和/或用DIDS对细胞进行预处理并没有在任何显著程度上改变红细胞膜对K+、Rb+和Cs+的通透性。实验结果支持这样的假说，即Li+和Na+，而不是K+、Rb+和Cs+，与CO3-- 形成单价带负电荷的离子对，这些离子对通过阴离子交换系统穿过膜。
