Soll A H
Am J Physiol. 1980 Apr;238(4):G366-75. doi: 10.1152/ajpgi.1980.238.4.G366.
Isolated canine gastric mucosal cells accumulate [14C]aminopyrine (AP) when treated with histamine, gastrin, and carbachol. In fractions of varying parietal cell content, this accumulation of AP correlated with the parietal cell content. Cimetidine caused parallel displacement of the dose-response curve to histamine, but failed to alter the response to carbachol or gastrin. Atropine caused parallel displacement of the dose-response curve to carbachol, but failed to inhibit the response to histamine or gastrin. The dissociation constants (Kb) for cimetidine inhibition of histamine and for atropine inhibition of carbachol were found to be 1.0 micro M and 1.3 nM, respectively, values comparable to those reported for other tissues. Thus, the isolated parietal cell appears to have pharmacologically typical H2- and muscarinic receptors, with gastrin acting at a third receptor site. Isobutyl methylxanthine (IMX) and the cAMP analogues dibutyryl cAMP (DBcAMP) and 8-bromo cAMP (but not the same analogues of cGMP) also stimulated AP accumulation. Atropine failed to inhibit the responses to IMX or DBcAMP, whereas cimetidine did inhibit the response to IMX, but not to DBcAMP.
分离的犬胃黏膜细胞在组胺、胃泌素和卡巴胆碱作用下会积累[14C]氨基比林（AP）。在壁细胞含量不同的组分中，AP的这种积累与壁细胞含量相关。西咪替丁使组胺的剂量反应曲线平行位移，但未能改变对卡巴胆碱或胃泌素的反应。阿托品使卡巴胆碱的剂量反应曲线平行位移，但未能抑制对组胺或胃泌素的反应。发现西咪替丁抑制组胺的解离常数（Kb）和阿托品抑制卡巴胆碱的解离常数分别为1.0微摩尔和1.3纳摩尔，这些值与其他组织报道的值相当。因此，分离的壁细胞似乎具有药理学上典型的H2和毒蕈碱受体，胃泌素作用于第三个受体位点。异丁基甲基黄嘌呤（IMX）以及环磷酸腺苷（cAMP）类似物二丁酰环磷腺苷（DBcAMP）和8-溴环磷腺苷（但不是环鸟苷酸（cGMP）的相同类似物）也刺激了AP的积累。阿托品未能抑制对IMX或DBcAMP的反应，而西咪替丁确实抑制了对IMX的反应，但不抑制对DBcAMP的反应。
