Thrasher T N, Jones R G, Keil L C, Brown C J, Ramsay D J
Am J Physiol. 1980 May;238(5):R340-5. doi: 10.1152/ajpregu.1980.238.5.R340.
Six dogs were administered third ventricular infusions of artificial cerebrospinal fluid (CSF) (292 mosmol/l) alone or artificial CSF to which neither NaCl, sucrose, glucose, or urea was added to yield a final osmolar concentration of 500 mosmol/l. The volume of water drunk during 45 min of infusion was measured and blood was sampled for determination of plasma vasopressin concentration at 15-min intervals. Artificial CSF made hypertonic by addition of NaCl or sucrose stimulated water intakes of 9.0 +/- 3.2 ml/kg (mean +/- SE) and 7.3 +/- 3.7 ml/kg, respectively. There was no statistical difference in the amounts drunk and the latencies. In contrast, artificial CSF containing glucose, urea, or artificial CSF alone were without effect. Plasma vasopressin concentration increased significantly in response to intraventricular NaCl and sucrose but was not affected by glucose, urea, or artificial CSF alone. These data are compatible with an osmoreceptor mechanism mediating drinking and vasopressin release.
给6只狗经第三脑室输注人工脑脊液（CSF）（292毫渗摩尔/升），或输注不含氯化钠、蔗糖、葡萄糖或尿素的人工脑脊液，以使最终渗透压浓度达到500毫渗摩尔/升。在输注的45分钟内测量饮水量，并每隔15分钟采集血液样本以测定血浆血管加压素浓度。通过添加氯化钠或蔗糖制成的高渗人工脑脊液分别刺激饮水量为9.0±3.2毫升/千克（平均值±标准误）和7.3±3.7毫升/千克。饮用量和潜伏期无统计学差异。相比之下，含葡萄糖、尿素的人工脑脊液或单纯人工脑脊液均无作用。脑室内注入氯化钠和蔗糖后，血浆血管加压素浓度显著升高，但单独的葡萄糖、尿素或人工脑脊液对其无影响。这些数据与介导饮水和血管加压素释放的渗透压感受器机制相符。
