短小芽孢杆菌紫外线照射孢子的抗性与恢复研究
Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus.
作者信息
Abshire R L, Bain B, Williams T
出版信息
Appl Environ Microbiol. 1980 Apr;39(4):695-701. doi: 10.1128/aem.39.4.695-701.1980.
A spore suspension model and a procedure for recovering ultraviolet (UV)-irradiated spores of Bacillus pumilus were investigated. A most-probable-number tube dilution method using double-strength Trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of UV energy. Aqueous suspensions of B. pumilus survived UV doses up to 108,000 ergs/mm2 as determined by a most-probable-number recovery and estimation procedure. Resistance and stability data were consistent and reproducible, indicating the dependability of this method for recovering UV-damaged spores. The procedures used to collect information concerning resistance characteristics for two strains of B. pumilus are discussed.
研究了短小芽孢杆菌的孢子悬浮液模型以及回收经紫外线（UV）照射的短小芽孢杆菌孢子的方法。发现使用双倍强度胰蛋白胨大豆肉汤的最大可能数试管稀释法在回收经亚致死剂量紫外线能量照射的最佳孢子数量方面优于琼脂平板法。通过最大可能数回收和估算程序确定，短小芽孢杆菌的水悬浮液在高达108,000尔格/平方毫米的紫外线剂量下仍能存活。抗性和稳定性数据一致且可重复，表明该方法在回收紫外线损伤孢子方面的可靠性。讨论了用于收集两株短小芽孢杆菌抗性特征信息的程序。
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