短小芽孢杆菌紫外线照射孢子的抗性与恢复研究

Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus.

作者信息

Abshire R L, Bain B, Williams T

出版信息

Appl Environ Microbiol. 1980 Apr;39(4):695-701. doi: 10.1128/aem.39.4.695-701.1980.

DOI:10.1128/aem.39.4.695-701.1980

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC291406/

Abstract

A spore suspension model and a procedure for recovering ultraviolet (UV)-irradiated spores of Bacillus pumilus were investigated. A most-probable-number tube dilution method using double-strength Trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of UV energy. Aqueous suspensions of B. pumilus survived UV doses up to 108,000 ergs/mm2 as determined by a most-probable-number recovery and estimation procedure. Resistance and stability data were consistent and reproducible, indicating the dependability of this method for recovering UV-damaged spores. The procedures used to collect information concerning resistance characteristics for two strains of B. pumilus are discussed.

摘要

研究了短小芽孢杆菌的孢子悬浮液模型以及回收经紫外线（UV）照射的短小芽孢杆菌孢子的方法。发现使用双倍强度胰蛋白胨大豆肉汤的最大可能数试管稀释法在回收经亚致死剂量紫外线能量照射的最佳孢子数量方面优于琼脂平板法。通过最大可能数回收和估算程序确定，短小芽孢杆菌的水悬浮液在高达108,000尔格/平方毫米的紫外线剂量下仍能存活。抗性和稳定性数据一致且可重复，表明该方法在回收紫外线损伤孢子方面的可靠性。讨论了用于收集两株短小芽孢杆菌抗性特征信息的程序。

相似文献

1

Resistance and recovery studies on ultraviolet-irradiated spores of Bacillus pumilus.短小芽孢杆菌紫外线照射孢子的抗性与恢复研究

Appl Environ Microbiol. 1980 Apr;39(4):695-701. doi: 10.1128/aem.39.4.695-701.1980.

2

Applicability of UV resistant Bacillus pumilus endospores as a human adenovirus surrogate for evaluating the effectiveness of virus inactivation in low-pressure UV treatment systems.抗紫外线短小芽孢杆菌芽孢作为人类腺病毒替代物在评估低压紫外线处理系统中病毒灭活效果方面的适用性。

J Microbiol Methods. 2016 Mar;122:43-9. doi: 10.1016/j.mimet.2016.01.012. Epub 2016 Jan 26.

3

Characterization of Bacillus pumilus E601 spores after single sublethal gamma irradiation treatments.短小芽孢杆菌E601孢子在单次亚致死剂量γ射线辐照处理后的特性研究

Appl Microbiol. 1975 Jan;29(1):34-9. doi: 10.1128/am.29.1.34-39.1975.

4

Increased radiation resistance of vegetative Bacillus pumilus.短小芽孢杆菌营养体的辐射抗性增强。

Appl Microbiol. 1974 Jul;28(1):41-6. doi: 10.1128/am.28.1.41-46.1974.

5

The radiation-induced inactivation of microorganisms in non-aqueous suspension: The effect of selected alcohols and paraffins on the radiation sensitivity of aerated Bacillus pumilus spores.非水悬浮液中微生物的辐射诱导失活：所选醇类和石蜡对充气短小芽孢杆菌孢子辐射敏感性的影响

Radiat Environ Biophys. 1981;19(2):109-16. doi: 10.1007/BF01324227.

6

Survival of spacecraft-associated microorganisms under simulated martian UV irradiation.航天器相关微生物在模拟火星紫外线照射下的存活情况。

Appl Environ Microbiol. 2005 Dec;71(12):8147-56. doi: 10.1128/AEM.71.12.8147-8156.2005.

7

Paradoxical DNA repair and peroxide resistance gene conservation in Bacillus pumilus SAFR-032.短小芽孢杆菌SAFR-032中矛盾的DNA修复与过氧化物抗性基因保守性

PLoS One. 2007 Sep 26;2(9):e928. doi: 10.1371/journal.pone.0000928.

8

Activation of Bacillus spores at moderately elevated temperatures (30-33 °C).在中等偏高温度（30-33°C）下激活芽孢杆菌孢子。

Antonie Van Leeuwenhoek. 2013 Mar;103(3):693-700. doi: 10.1007/s10482-012-9839-3. Epub 2012 Nov 7.

9

Alterations in radiation resistance of Bacillus pumilus E601 spores.短小芽孢杆菌E601孢子辐射抗性的改变。

Radiat Res. 1977 Feb;69(2):367-75.

10

Spore UV and acceleration resistance of endolithic Bacillus pumilus and Bacillus subtilis isolates obtained from Sonoran desert basalt: implications for lithopanspermia.从索诺兰沙漠玄武岩中分离出的内生短小芽孢杆菌和枯草芽孢杆菌的孢子对紫外线和加速条件的抗性：对岩石泛种论的影响

Astrobiology. 2003 Winter;3(4):709-17. doi: 10.1089/153110703322736033.

引用本文的文献

1

High stringency evaluation of the inactivation / exclusion efficacy of a MALDI-TOF MS chemical extraction method, with filtration of extract through 0.1 µm filters, on Bacillus anthracis Vollum vegetative cells and spores.采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）化学提取法，并通过0.1 µm滤器过滤提取物，对炭疽芽孢杆菌沃勒姆株营养细胞和芽孢的灭活/排除效果进行高严格度评估。

PLoS One. 2017 May 8;12(5):e0177294. doi: 10.1371/journal.pone.0177294. eCollection 2017.

2

Resistance of selected strains of Pseudomonas aeruginosa to low-intensity ultraviolet radiation.铜绿假单胞菌特定菌株对低强度紫外线辐射的抗性

Appl Environ Microbiol. 1981 Jun;41(6):1419-23. doi: 10.1128/aem.41.6.1419-1423.1981.

本文引用的文献

1

Thymine Photoproducts but not Thymine Dimers Found in Ultraviolet-Irradiated Bacterial Spores.在紫外辐射的细菌孢子中发现胸腺嘧啶光产物而不是胸腺嘧啶二聚体。

Science. 1965 Jul 16;149(3681):308-10. doi: 10.1126/science.149.3681.308.

2

RECOVERY FROM ULTRAVIOLET IRRADIATION IN ESCHERICHIA COLI.大肠杆菌中紫外线照射后的恢复

J Bacteriol. 1949 Mar;57(3):363-75. doi: 10.1128/jb.57.3.363-375.1949.

3

Factors which Influence the Growth of Heat-treated Bacteria: I. A Comparison of Four Agar Media.影响热处理细菌生长的因素：I. 四种琼脂培养基的比较

J Bacteriol. 1943 Apr;45(4):395-403. doi: 10.1128/jb.45.4.395-403.1943.

4

The Importance of Enrichments in the Cultivation of Bacterial Spores Previously Exposed to Lethal Agencies.富集培养对先前暴露于致死因素的细菌芽孢的重要性。

J Bacteriol. 1937 Aug;34(2):179-89. doi: 10.1128/jb.34.2.179-189.1937.

5

GAMMA IRRADIATION OF BACILLUS SUBTILIS SPORES IN THE PRESENCE OF SUGARS.在糖类存在的情况下对枯草芽孢杆菌孢子进行伽马辐照。

J Gen Microbiol. 1964 Feb;34:185-93. doi: 10.1099/00221287-34-2-185.

6

INACTIVATION OF DRIED BACTERIA AND BACTERIAL SPORES BY MEANS OF IONIZING RADIATION.利用电离辐射使干燥细菌及细菌芽孢失活

Acta Pathol Microbiol Scand. 1964;60:253-64. doi: 10.1111/apm.1964.60.2.253.

7

Relative resistances of micro-organisms to cathode rays. III. Bacterial spores.微生物对阴极射线的相对抗性。III. 细菌芽孢

Appl Microbiol. 1956 May;4(3):149-52. doi: 10.1128/am.4.3.149-152.1956.

8

Effects of continuous and interrupted radiation on microorganisms.连续辐射和间断辐射对微生物的影响。

Appl Microbiol. 1967 Jul;15(4):785-9. doi: 10.1128/am.15.4.785-789.1967.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。