Schäfer R, Schwartz A C
Arch Microbiol. 1980 Jan;124(1):111-4. doi: 10.1007/BF00407038.
Resting cells of Clostridium sticklandii took up thymine or uracil, when grown in a medium containing 40 mM serine and 20 mM thymine or uracil. The uptake was much lower, when the cells had been grown in a complex medium. Cell-free extracts from cells grown in the complex medium reduced the two bases to the dihydro compounds and decomposed dihydrothymine to beta-ureidoisobutyrate, as indicated by thin-layer chromatography. Uptake and degradation were stimulated by both NADH and NADPH. Further breakdown did not occur, as 14CO2 was not evolved from C-2-labelled thymine or uracil. The rates of pyrimidine uptake and breakdown of C. sticklandii were lower than those reported for C. sporogenes (Hilton et al., 1975).
当在含有40 mM丝氨酸和20 mM胸腺嘧啶或尿嘧啶的培养基中生长时，斯氏梭菌的静息细胞会摄取胸腺嘧啶或尿嘧啶。当细胞在复合培养基中生长时，摄取量要低得多。如薄层色谱所示，从在复合培养基中生长的细胞中提取的无细胞提取物将这两种碱基还原为二氢化合物，并将二氢胸腺嘧啶分解为β-脲基异丁酸酯。NADH和NADPH均刺激摄取和降解。由于未从C-2标记的胸腺嘧啶或尿嘧啶中释放出14CO2，因此未发生进一步分解。斯氏梭菌嘧啶摄取和分解的速率低于产芽孢梭菌报道的速率（希尔顿等人，1975年）。
