Horisberger M A, de Staritzky C, Content J
Arch Virol. 1980;64(1):9-16. doi: 10.1007/BF01317386.
The intracellular glycosylation of influenza virus hemagglutinin has been studied in calf kidney primary cultures infected with influenza virus A/NWS (H0N1). Intermediate glycosylated forms were obtained by the addition of glucosamine to the culture medium; they were characterized after [35S]-methionine labelling by their precursor relationship, by immunoprecipitation and peptide mapping. The underglycosylated hemagglutinins were distributed in classes of discrete molecular weight indicating that glycosylation occurred in a stepwise manner. A total of 8 different bands, suggesting 7 glycosylation sites, was obtained under conditions of both complete and incomplete glycosylation.
对感染甲型流感病毒A/NWS（H0N1）的犊牛肾原代培养物中流感病毒血凝素的细胞内糖基化进行了研究。通过向培养基中添加葡糖胺获得了中间糖基化形式；在进行[35S] - 甲硫氨酸标记后，通过它们的前体关系、免疫沉淀和肽图谱对其进行了表征。低糖基化的血凝素分布在离散分子量类别中，表明糖基化是以逐步方式发生的。在完全和不完全糖基化条件下均获得了总共8条不同的条带，提示有7个糖基化位点。
