Cass A E, Hill A O, Smith B E
Biochem J. 1977 Sep 1;165(3):587-9. doi: 10.1042/bj1650587.
The C-2 proton of one histidine residue in bovine erythrocyte superoxide dismutase is shown to be particularly labile. This residue is identified by tritiation, protein digestion and subsequent peptide 'mapping' as histidine-41. A half-life for the exchange of histidine C-2 1H for 2H in 2H2O as solvent, at pD 8.1 and 40 degrees C, is estimated as approx. 9.2h, by 1H nuclear-magnetic-resonance spectroscopy.
已表明牛红细胞超氧化物歧化酶中一个组氨酸残基的C-2质子特别不稳定。通过氚化、蛋白质消化及随后的肽“图谱分析”，将该残基鉴定为组氨酸-41。在pD 8.1和40℃条件下，以重水为溶剂，通过氢核磁共振光谱法估计，组氨酸C-2位的氢与重氢交换的半衰期约为9.2小时。
