Merryweather M J, Knowler J T
Biochem J. 1980 Feb 15;186(2):405-10. doi: 10.1042/bj1860405.
The kinetics of the synthesis of the components of polyribosomes was investigated in the uterus of the immature rat responding to the administration of oestradiol-17 beta. The hormone brings about a rapid stimulation of the association of newly synthesized mRNA with uterine ribosomes, which is maximal 2-4 h after oestradiol administration and causes the aggregation of pre-existing ribosomes into polyribosomes. Despite the striking stimulation of rRNA synthesis 2-4 h after hormone treatment [Knowler & Smellie (1971) Biochem. J. 125, 605-614], the accumulation of new rRNA into ribosomes does not reach a peak until 12 h after administration. At this time, the incorporation of new ribosomal protein is also maximal. A second peak of incorporation of newly synthesized mRNA into polyribosomes follows the peak of ribosome synthesis and coincides with the oestrogen-activated synthesis of DNA.
在对未成熟大鼠子宫给予17β-雌二醇后，研究了多核糖体组分合成的动力学。该激素能迅速刺激新合成的mRNA与子宫核糖体的结合，在给予雌二醇后2 - 4小时达到最大值，并使预先存在的核糖体聚集成多核糖体。尽管在激素处理后2 - 4小时rRNA合成受到显著刺激[诺勒和斯梅利（1971年）《生物化学杂志》125卷，605 - 614页]，但新rRNA积累到核糖体中直到给药后12小时才达到峰值。此时，新核糖体蛋白的掺入也达到最大值。新合成的mRNA掺入多核糖体的第二个峰值出现在核糖体合成峰值之后，并与雌激素激活的DNA合成同时出现。
