不同激素状态大鼠子宫mRNA的多样性和复杂性。
Diversity and complexity of uterine mRNA from rats of differing hormonal states.
作者信息
Aziz S, Balmain A, Knowler J T
出版信息
Eur J Biochem. 1979 Oct;100(1):85-94. doi: 10.1111/j.1432-1033.1979.tb02036.x.
The diversity and complexity of the uterine mRNA population has been compared at two different stages of uterine growth and development in the rat. Analysis by cDNA hybridization to homologous mRNA indicates that there are 8000 different sequences expressed in the immature rat uterus responding to just 4 h of oestradiol-17 beta-induced growth while the fully developed uterus expresses 36 000 sequences. As a check on our results, the complexity is re-analysed by hybridization of mercurated mRNA to total unique DNA. Analysis by this method indicates that the hormone-stimulated immature rat uterus contains 12 000 poly(A)-containing mRNA sequences while the fully differentiated adult tissue contains 53 000 diverse sequences.
在大鼠子宫生长和发育的两个不同阶段，对子宫mRNA群体的多样性和复杂性进行了比较。通过与同源mRNA的cDNA杂交分析表明，未成熟大鼠子宫在仅4小时的雌二醇-17β诱导生长后，有8000种不同序列表达，而完全发育的子宫则表达36000种序列。作为对我们结果的检验，通过将汞化mRNA与总独特DNA杂交来重新分析复杂性。用这种方法分析表明，激素刺激的未成熟大鼠子宫含有12000种含poly(A)的mRNA序列，而完全分化的成年组织含有53000种不同序列。
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