Reisner H M, Strand E A, Chung K S, Roberts H R
Br J Haematol. 1980 Feb;44(2):313-21. doi: 10.1111/j.1365-2141.1980.tb01214.x.
An agarose plate method for detecting human alloantibodies (inhibitors) to coagulant factor IX has been developed. The assay is based on the ability of such antisera to inhibit the coagulation of a mixture of haemophilia B plasma, normal plasma and platelet subsitute in an agarose matrix. The agarose plate method was also adopted to measure levels of FIX antigen (IX:Ag) in plasma. Using this technique, 12 of 17 obligate carriers of haemophilia B demonstrated reduced levels of IX:Ag. Three of the five carriers with normal IX:Ag levels were members of kindred in which affected individuals had normal or near normal levels of IX:Ag.
已开发出一种用于检测人抗凝血因子IX同种抗体（抑制剂）的琼脂糖平板法。该检测基于此类抗血清在琼脂糖基质中抑制B型血友病血浆、正常血浆和血小板替代物混合物凝血的能力。琼脂糖平板法也被用于测量血浆中FIX抗原（IX:Ag）的水平。使用该技术，17名B型血友病 obligate携带者中有12名显示IX:Ag水平降低。5名IX:Ag水平正常的携带者中有3名是受影响个体IX:Ag水平正常或接近正常的家族成员。
