Browning K S, Leung D W, Clark J M
Biochemistry. 1980 May 13;19(10):2276-83. doi: 10.1021/bi00551a044.
Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5' terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) ribonucleic acid (RNA) to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat gern 80S ribosomes convey partial protection to a region 20-52 nucleotides from the 5' terminus of STNV RNA and convey protection against high levels of ribonucleases to a region 20-44 nucleotides from the 5' terminus of STNV RNA. Wheat germ 40S ribosomes convey partial protection to a region 3-52 nucleotides from the 5' terminus of STNV RNA and convey protection against high levels of ribonucleases to a region 10-47 nucleotides from the 5' terminum of STNV RNA. Characterization of these levels of protection against attack by specific ribonucleases establishes that wheat germ 40S and 80S ribosomes form initiation complexes with a linear conformation of STNV RNA lacking the 5'-terminal stem and loop anticipated by Leung and co-workers [Leung, D. W., Browning, K. S., Heckman, J. E., RajBhandary, U. L., & Clark, J. M., Jr. (1979) Biochemistry 18, 1361-1366] for the 5' terminus of STNV RNA.
小麦胚芽核糖体与体外放射性碘化的卫星烟草坏死病毒（STNV）核糖核酸（RNA）5'末端第30 - 32位的AUG密码子结合，形成起始复合物，该复合物可保护RNA的特定区域免受核糖核酸酶的攻击。小麦胚芽80S核糖体对STNV RNA 5'末端20 - 52个核苷酸的区域提供部分保护，并对STNV RNA 5'末端20 - 44个核苷酸的区域提供针对高浓度核糖核酸酶的保护。小麦胚芽40S核糖体对STNV RNA 5'末端3 - 52个核苷酸的区域提供部分保护，并对STNV RNA 5'末端10 - 47个核苷酸的区域提供针对高浓度核糖核酸酶的保护。对这些针对特定核糖核酸酶攻击的保护水平的表征表明，小麦胚芽40S和80S核糖体与STNV RNA的线性构象形成起始复合物，该线性构象缺乏梁等人[梁，D. W.，布朗宁，K. S.，赫克曼，J. E.，拉杰班达里，U. L.，& 克拉克，J. M.，Jr.（1979年）生物化学杂志18卷，第1361 - 1366页]所预测的STNV RNA 5'末端的茎环结构。
