Fulford A J, Peel W E
Biochim Biophys Acta. 1980 May 23;598(2):237-46. doi: 10.1016/0005-2736(80)90002-4.
A theoretical model is proposed which states that the time-independent fluorescence anisotropy of the rod-shaped molecule diphenylhexatriene incorporated into lipid bilayers is a direct result of forces constraining the diphenyl-hexatriene molecule. These forces are postulated as equating with the lateral pressure operating within the bilayer independently of the probe molecule. Insertion into the model of experimental observations (recorded in the literature) on anisotropy of diphenylhexatriene in lipid bilayers as a function of temperature yielded values of lateral pressure, which decreased with temperature, and sharply at the temperature defining the transition from gel phase to fluid phase. The values so predicted for the mid-point of the transition and for the entirely fluid phase, respectively, compared favourably with estimates of the lateral pressures in these physical states, that have been reported elsewhere and arrived at either from theories describing lipid chain behaviour or from lipid monolayer compression experiments. Previously documented effects on anisotropy induced by incorporation of cholesterol into fluid lipid bilayers have been interpreted as reflections of rises in intramembranal lateral pressure.
提出了一个理论模型，该模型指出，掺入脂质双层中的棒状分子二苯基己三烯的与时间无关的荧光各向异性是限制二苯基己三烯分子的力的直接结果。这些力被假定等同于在双层内起作用的横向压力，与探针分子无关。将关于脂质双层中二苯基己三烯各向异性随温度变化的实验观察结果（记录在文献中）代入该模型，得出了横向压力值，该值随温度降低，并在定义从凝胶相到流体相转变的温度处急剧下降。分别为转变中点和完全流体相预测的值，与在其他地方报道的、通过描述脂质链行为的理论或脂质单层压缩实验得出的这些物理状态下的横向压力估计值相比，结果良好。先前记录的将胆固醇掺入流体脂质双层中对各向异性的影响，已被解释为膜内横向压力升高的反映。
