在细胞培养中对γ-氨基丁酸（GABA）作为哺乳动物皮层神经元抑制性递质的生理学鉴定。

Physiological identification of GABA as the inhibitory transmitter for mammalian cortical neurons in cell culture.

作者信息

Dichter M A

出版信息

Brain Res. 1980 May 19;190(1):111-21. doi: 10.1016/0006-8993(80)91163-4.

Abstract

(1) Rat cortical neurons grown in dissociated cell culture exhibit IPSPs which appear to be generated by an increase in membrane conductance to chloride. (2) The neurons are all sensitive to GABA in micromolar concentrations and GABA mimics the inhibitory transmitter. (3) The neurons are much less sensitive to glycine and insensitive to taurine. (4) Bicuculline and strychnine both block essentially all IPSPs and at the same concentrations block GABA effects. (5) It is concluded that GABA is the main, or only, inhibitory transmitter utilized by the cortical neurons in vitro. The relevance of this conclusion to in situ transmitter identification is discussed.

摘要

(1) 在解离细胞培养中生长的大鼠皮层神经元表现出抑制性突触后电位（IPSPs），这些电位似乎是由氯离子膜电导增加所产生的。(2) 这些神经元对微摩尔浓度的γ-氨基丁酸（GABA）均敏感，且GABA可模拟抑制性递质。(3) 这些神经元对甘氨酸的敏感性要低得多，对牛磺酸不敏感。(4) 荷包牡丹碱和士的宁基本上能阻断所有的抑制性突触后电位，且在相同浓度下能阻断GABA的作用。(5) 得出的结论是，GABA是体外培养的皮层神经元所利用的主要或唯一的抑制性递质。讨论了这一结论与原位递质鉴定的相关性。