Blume H W, Pittman Q J, Renaud L P
Brain Res. 1981 Mar 23;209(1):145-58. doi: 10.1016/0006-8993(81)91177-x.
Medial hypothalamic neurons in pentobarbital anesthetized rats were identified by location and response to electrical stimulation of the amygdala, medial preoptic area, midbrain periaqueductal gray and median eminence. Cells were then examined for their sensitivity to microiontophoretic applications of GABA, glycine and 3 related amino acids, i.e. beta-guanidinopropionic acid, delta-aminovaleric acid and beta-alanine. Application of all agents decreased the spontaneous and glutamate or aspartate evoked activity of the majority of neurons in all identified categories. The majority of neurons were more sensitive to beta-guanidinopropionate, delta aminovalerate and GABA than to glycine and beta-alanine. Bicuculline and picrotoxin produced a selective and reversible antagonism of depressions evoked by GABA and GABA-like amino acids whereas strychnine produced a selective and reversible antagonism of depressions evoked by glycine and beta-alanine. Bicuculline and picrotoxin, but not strychnine, were observed to diminish synaptic inhibition evoked by electrical stimulation of several sites when these agents were administered by microiontophoresis and by i.v. injections in convulsive doses. These observations suggest that many medial hypothalamic neurons have both GABA and glycine receptors but that GABA may have the more important role as a neurotransmitter common to afferent or recurrent inhibitory hypothalamic pathways.
通过位置以及对杏仁核、内侧视前区、中脑导水管周围灰质和正中隆起进行电刺激的反应，鉴定了戊巴比妥麻醉大鼠下丘脑内侧的神经元。然后检测这些细胞对微量离子电泳施加的GABA、甘氨酸和3种相关氨基酸（即β-胍基丙酸、δ-氨基戊酸和β-丙氨酸）的敏感性。所有试剂的施加均降低了所有已鉴定类别中大多数神经元的自发活动以及谷氨酸或天冬氨酸诱发的活动。大多数神经元对β-胍基丙酸盐、δ-氨基戊酸盐和GABA的敏感性高于对甘氨酸和β-丙氨酸的敏感性。荷包牡丹碱和印防己毒素对GABA和GABA样氨基酸诱发的抑制产生选择性和可逆性拮抗作用，而士 的宁对甘氨酸和β-丙氨酸诱发的抑制产生选择性和可逆性拮抗作用。当通过微量离子电泳和静脉注射惊厥剂量给予这些试剂时，观察到荷包牡丹碱和印防己毒素而非士的宁会减弱由几个部位的电刺激诱发的突触抑制。这些观察结果表明，许多下丘脑内侧神经元同时具有GABA和甘氨酸受体，但GABA作为传入或下丘脑反复抑制性通路共有的神经递质可能具有更重要的作用。
