大鼠脑突触体高亲和力系统对γ-氨基丁酸的净摄取
Net uptake of gamma-aminobutyric acid by a high-affinity system of rat brain synaptosomes.
作者信息
Pastuszko A, Wilson D F, Erecińska M
出版信息
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1242-4. doi: 10.1073/pnas.78.2.1242.
Abstract
Rat brain synaptosomes isolated on discontinuous Ficoll gradient carry out rapid net uptake of gamma-aminobutyric acid through a high-affinity system (Km = 6.25 microM; Vmax = 1.2 nmol/min per mg of protein). The uptake of the labeled neurotransmitter is dependent on sodium concentration and is abolished by addition of 40 microM veratridine or 0.5 mM 2,4-diaminobutyric acid. Homoexchange in this preparation accounts for less than 10% of the measured uptake of gamma-amino[14C]butyric acid. It is concluded that the high-affinity transport exhibits properties characteristic of a system that is responsible for the rapid removal of gamma-aminobutyric acid from the synaptic cleft after neuronal transmission.
摘要
通过不连续的Ficoll梯度分离得到的大鼠脑突触体，通过高亲和力系统（Km = 6.25微摩尔；Vmax = 1.2纳摩尔/分钟/毫克蛋白质）对γ-氨基丁酸进行快速净摄取。标记神经递质的摄取依赖于钠浓度，并可通过添加40微摩尔藜芦碱或0.5毫摩尔2,4-二氨基丁酸而被消除。在该制剂中，同源交换占γ-[14C]氨基丁酸测量摄取量的不到10%。得出的结论是，高亲和力转运表现出一种系统的特性，该系统负责在神经元传递后从突触间隙快速清除γ-氨基丁酸。
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