Rasilo M L
Can J Biochem. 1980 Apr;58(4):281-6. doi: 10.1139/o80-037.
Human teratocarcinoma derived cells, line PA 1, were labeled with radioactive monosaccharides and subsequently digested with pronase. Large sized glycopeptides (fraction A) were isolated by gel filtration on Bio-Gel P-10. Their chromatography on concanavalin A-Sepharose gave three subfractions, two of which were eluted with a sugar-free buffer and the third with 10 mM alpha-methyl mannoside. The first subfraction (fraction A-Con A Ia) incorporated label from [3H]galactose and [3H]glucosamine and contained the largest components of fraction A. The second and the third subfractions (fractions A-Con A Ib and A-Con A II) were glycopeptides which incorporated label from tritiated fucose, mannose, galactose, and glucosamine. Even these molecules were of large size eluting partially at the void volume from Bio-Gel P-60. The glycopeptides of fraction A-Con A Ib contained mannose, fucose, galactose, N-acetylglucosamine, and N-acetylgalactosamine. Fucose and galactose residues occupied ultimate or penultimate positions at the nonreducing termini of the oligosaccharides. N-Acetylneuraminic acid, too, was present in the glycopeptides of fraction A.
人畸胎瘤衍生细胞系PA 1用放射性单糖标记，随后用链霉蛋白酶消化。通过在Bio-Gel P-10上进行凝胶过滤分离出大尺寸糖肽（组分A）。它们在伴刀豆球蛋白A-琼脂糖上进行色谱分析得到三个亚组分，其中两个用无糖缓冲液洗脱，第三个用10 mM α-甲基甘露糖苷洗脱。第一个亚组分（组分A-Con A Ia）掺入了来自[3H]半乳糖和[3H]葡糖胺的标记，并且包含组分A中最大的成分。第二个和第三个亚组分（组分A-Con A Ib和A-Con A II）是糖肽，它们掺入了来自氚化岩藻糖、甘露糖、半乳糖和葡糖胺的标记。即使这些分子尺寸较大，在Bio-Gel P-60上也部分在空体积处洗脱。组分A-Con A Ib的糖肽含有甘露糖、岩藻糖、半乳糖、N-乙酰葡糖胺和N-乙酰半乳糖胺。岩藻糖和半乳糖残基占据寡糖非还原末端的最终或倒数第二个位置。N-乙酰神经氨酸也存在于组分A的糖肽中。
